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Thierry DOUKI

laboratory Molecular Systems and Nanomaterials for Energy and Health(SyMMES) UMR CNRS/UGA/CEA
team Chemistry at the interface with Biology in Environment, Health and Toxicology (CIBEST)

tel.jpg 04.38.78.31.91
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Functions:

Scientisit, deputy head of the laboratory

 

Research Interests

- Characterization of DNA damage induced by chemical agents (Polycyclic aromatic hydrocarbons, alkylating agents, agents alkylants, reactive oxygen species) and radiation (ultraviolet, ionizing).

- Development of analytical tools for the quantification of these damage.

- Study of the formation and repair at the cellular level after exposure to various stresses.

 

CV


Training
- HDR, Grenoble University (1999)

- PhD in chemistry, Grenoble University (1992)

- Diploma of the Chemistry Engineering School of Lyon (1989)

- Master in organic synthesis, Lyon University (1989)


Professionnal experience

- 2013-2016: head of the Laboratory of Biological and Inorganic Chemsitry

- 2007-2012: Head of the "Nucleic Acids Lesions" team

- 1993-       : Permanent scientist in the "Nucleic Acids Lesions" team

- 1992-1993: post-doctoral fellow at University of California in Berkeley; Prof. Bruce Ames' group
                    (DNA damage as biomarkers of endogenous processes)

- 1989-1992: Science thesis, supervised by Jean Cadet ("Nucleic Acids Lesions" team)
                     (Study of the photochemistry of isolated DNA and of model compounds)

Awards

- 2018: "Research Award" of the American Society for Photobiology

- 2006: "New Investigator Award" of the AmericanSociety for Photobiology

- 1994: Young scientist award of the French Society for Photobiology      

 

Publications

Co-author of more than 180 original articles , and of more than 60 review articicles and book chapters (H-index 61, ISI WoK).

List of publications.pdf

 

Selection of publications:

(1) Youssef, A., von Koschembahr, A., Caillat, S., Corre, S., Galibert, M. D. and Douki, T. (2018) 6-Formylindolo[3,2-b]carbazole (FICZ) is a Very Minor Photoproduct of Tryptophan at Biologically Relevant Doses of UVB and Simulated Sunlight. Photochem. Photobiol. in press.

(2) von Koschembahr, A., Youssef, A., Béal, D., Calissi, C., Bourgart, E., Marques, M., Leccia, M.-T., Giot, J.-P., Maitre, A. and Douki, T. (2018) Solar simulated light exposure alters metabolization and genotoxicity induced by benzo[a]pyrene in human skin. Sci. Rep. In Press.

(3) Douki, T., Corbiere, C., Preterre, D., Martin, P. J., Lecureur, V., Andre, V., Landkocz, Y., Pottier, I., Keravec, V., Fardel, O., Moreira-Rebelo, S., Pottier, D., Vendeville, C., Dionnet, F., Gosset, P., Billet, S., Monteil, C. and Sichel, F. (2018) Comparative study of diesel and biodiesel exhausts on lung oxidative stress and genotoxicity in rats. Environ. Pollut. 235, 514-524.

(4) Douki, T., von Koschembahr, A. and Cadet, J. (2017) Insight in DNA repair of UV-induced pyrimidine dimers by chromatographic methods. Photochem. Photobiol. 93, 207-215.

(5) Genies, C., Jullien, A., Lefebvre, E., Revol, M., Maitre, A. and Douki, T. (2016) Inhibition of the formation of benzo a pyrene adducts to DNA in A549 lung cells exposed to mixtures of polycyclic aromatic hydrocarbons. Toxicol. Vitro 35, 1-10.

(6) Douki, T. and Sage, E. (2016) Dewar valence isomers, the third type of environmentally relevant DNA photoproducts induced by solar radiation. Photochem. Photobiol. Sci. 15, 24-30.

(7) Douki, T. (2016) Sunlight-induced DNA damage: molecular mechanisms and photoprotection strategies, In Skin Stress Response Pathways: Environmental Factors and Molecular Opportunities (Wondrak, G. T., Ed.) pp 49-77, Springer Int. Publish.

(8) Douki, T. (2016) Relative Contributions of UVB and UVA to the Photoconversion of (6-4) Photoproducts into their Dewar Valence Isomers. Photochem. Photobiol. 92, 587-594.

(9) Banyasz, A., Esposito, L., Douki, T., Perron, M., Lepori, C., Improta, R. and Markovitsi, D. (2016) Effect of C5-Methylation of Cytosine on the UV-Induced Reactivity of Duplex DNA: Conformational and Electronic Factors. J. Phys. Chem. B 120, 4232-4242.

(10) Premi, S., Wallisch, S., Mano, C. M., Weiner, A. B., Bacchiocchi, A., Wakamatsu, K., Bechara, E. J. H., Halaban, R., Douki, T. and Brash, D. E. (2015) Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure. Science 347, 842-847.

(11) Douki, T., Meador, J. A., Berard, I. and Wack, A. (2015) N4-Methylation of Cytosine Drastically Favors the Formation of (6-4) Photoproducts in a TCG Context. Photochem. Photobiol. 91, 102-108.

(12) Batal, M., Rebelo-Moreira, S., Hamona, N., Bayle, P. A., Mouret, S., Clery-Barraud, C., Boudry, I. and Douki, T. (2015) A guanine-ethylthioethyl-glutathione adduct as a major DNA lesion in the skin and in organs of mice exposed to sulfur mustard. Toxicol. Lett. 233, 1-7.

(13) Bacqueville, D., Douki, T., Duprat, L., Rebelo-Moreira, S., Guiraud, B., Dromigny, H., Perier, V., Bessou-Touya, S. and Duplan, H. (2015) A new hair follicle-derived human epidermal model for the evaluation of sunscreen genoprotection. J. Photochem. Photobiol. B: Biol. 151, 31-38.

(14) Meador, J. A., Baldwin, A. J., Pakulski, J. D., Jeffrey, W. H., Mitchell, D. L. and Douki, T. (2014) The significance of the Dewar valence photoisomer as a UV radiation-induced DNA photoproduct in marine microbial communities. Environ. Microbiol. 16, 1808-1820.

(15) Batal, M., Boudry, I., Mouret, S., Clery-Barraud, C., Wartelle, J., Berard, I. and Douki, T. (2014) DNA damage in internal organs after cutaneous exposure to sulphur mustard. Toxicol. Appl. Pharmacol. 278, 39-44.

(16) Genies, C., Maitre, A., Lefebvre, E., Jullien, A., Chopard-Lallier, M. and Douki, T. (2013) The Extreme Variety of Genotoxic Response to Benzo a pyrene in Three Different Human Cell Lines from Three Different Organs. PLoS One 8, 11.

(17) Batal, M., Boudry, I., Mouret, S., Wartelle, J., Emorine, S., Bertoni, M., Berard, I., Clery-Barraud, C. and Douki, T. (2013) Temporal and spatial features of the formation of DNA adducts in sulfur mustard-exposed skin. Toxicol. Appl. Pharmacol. 273, 644-650.

(18) Vaya, I., Gustavsson, T., Douki, T., Berlin, Y. and Markovitsi, D. (2012) Electronic: Excitation Energy Transfer between Nucleobases of Natural DNA. J. Am. Chem. Soc. 134, 11366-11368.

(19) Tarantini, A., Maitre, A., Lefebvre, E., Marques, M., Rajhi, A. and Douki, T. (2011) Polycyclic aromatic hydrocarbons in binary mixtures modulate the efficiency of benzo a pyrene to form DNA adducts in human cells. Toxicology 279, 36-44.

(20) Tarantini, A., Douki, T., Personnaz, M. B., Besombes, J. L., Jafrezzo, J. L. and Maitre, A. (2011) Effect of the chemical composition of organic extracts from environmental and industrial atmospheric samples on the genotoxicity of polycyclic aromatic hydrocarbons mixtures. Toxicol Environ. Chem. 93, 941-954.

(21) Noonan, F. P., Zaidi, M. R., Wolnicka-Glubisz, A., Anver, M. R., Bahn, J., Wielgus, A., Cadet, J., Douki, T., Mouret, S., Popratiloff, A., Merlino, G. and De Fabo, E. C. (2011) Two UV pathways to melanoma. Pigment Cell Melanoma Res. 24, 799-799.

(22) Mouret, S., Leccia, M. T., Bourrain, J. L., Douki, T. and Beani, J. C. (2011) Individual Photosensitivity of Human Skin and UVA-Induced Pyrimidine Dimers in DNA. J. Invest. Dermatol. 131, 1539-1546.

(23) Mouret, S., Bogdanowicz, P., Haure, M. J., Castex-Rizzi, N., Cadet, J., Favier, A. and Douki, T. (2011) Assessment of the Photoprotection Properties of Sunscreens by Chromatographic Measurement of DNA Damage in Skin Explants. Photochem. Photobiol. 87, 109-116.

(24) Banyasz, A., Vaya, I., Changenet-Barret, P., Gustavsson, T., Douki, T. and Markovitsi, D. (2011) Base Pairing Enhances Fluorescence and Favors Cyclobutane Dimer Formation Induced upon Absorption of UVA Radiation by DNA. J. Am. Chem. Soc. 133, 5163-5165.

(25) Mouret, S., Philippe, C., Gracia-Chantegrel, J., Banyasz, A., Karpati, S., Markovitsi, D. and Douki, T. (2010) UVA-induced cyclobutane pyrimidine dimers in DNA: a direct photochemical mechanism? Org. Biomol. Chem. 8, 1706-1711.

(26) Falletti, O. and Douki, T. (2008) Low glutathione level favors formation of DNA adducts to 4-hydroxy-2(E)-nonenal, a major lipid peroxidation product. Chem. Res. Toxicol. 21, 2097-2105.

(27) Falletti, O., Cadet, J., Favier, A. and Douki, T. (2007) Trapping of 4-hydroxynonenal by glutathione efficiently prevents formation of DNA adducts in human cells. Free Radic. Biol. Med. 42, 1258-1269.

(28) Mouret, S., Baudouin, C., Charveron, M., Favier, A., Cadet, J. and Douki, T. (2006) Cyclobutane pyrimidine dimers are predominant DNA lesions in whole human skin exposed to UVA radiation. Proc. Natl. Acad. Sci. U. S. A. 103, 13765-13770.

(29) Douki, T., Ravanat, J. L., Pouget, J. P., Testard, I. and Cadet, J. (2006) Minor contribution of direct ionization to DNA base damage induced by heavy ions. Int. J. Radiat. Biol. 82, 119-127.

(30) Douki, T. (2006) Low ionic strength reduces cytosine photoreactivity in UVC-irradiated isolated DNA. Photochem. Photobiol. Sci. 5, 1045-1051.

(31) Douki, T. (2006) Effect of denaturation on the photochemistry of pyrimidine bases in isolated DNA. J. Photochem. Photobiol. B: Biol. 82, 45-52.

(32) Chandor, A., Berteau, O., Douki, T., Gasparutto, D., Sanakis, Y., Ollagnier-De-Choudens, S., Atta, M. and Fontecave, M. (2006) Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis. J. Biol. Chem. 281, 26922-26931.

(33) Douki, T., Reynaud-Angelin, A., Cadet, J. and Sage, E. (2003) Bipyrimidine photoproducts rather than oxidative lesions are the main type of DNA damage involved in the genotoxic effect of solar UVA radiation. Biochemistry 42, 9221-9226.

 

 

 

Teaching

Lectures on chemical carcinogenesis, effects of UV radiation, mass spectrometry and oxidative stress

Other

- Associate editor for Photochemistry and Photobiology

- Member of the European Society for Photobiology (executive committee 2005-2007)

- Member of the American and European Societies for Photobiology (council member since 2014)

- Secretary of the French Society for Photobiology

- Member of the French Society for Genetic Toxicology

- Expert in the "Physical Agents" group of the National Agency of Heatlh and Environmental Safety (ANSES)

 

 

 


 

 

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