Nanoparticle dispersion for toxicology assessment

Results of nanotoxicology testing have suffered from the lack of accurate physico-chemical characterization of nanomaterials, and of the use of uncontrolled, non-reproducible nanomaterial dispersing procedures. In collaboration with Laboratoire Francis Perrin (CEA Saclay), we have been involved for long in the development standard operating procedures using ultrasonication for the dispersion of nanomaterials.

Nanoparticle dispersion for toxicology assessment

The cuphorn device.

Our procedures either use a classical ultrasonic probe or the less common cuphorn device. Nanomaterials are dispersed either in ultrapure water or in a diluted protein solution such as bovine serum albumin. High energy ultrasound is applied to these suspensions, either in continuous or in pulsed mode, under controlled temperature. After sonication, these NP suspensions are diluted in exposure medium, i.e. either cell culture medium or hydroponic solution or bacterial growth medium.


Our results show that the toxicological outcome of NPs varies with respect to the dispersion procedure (Carrière et al., 2014).



M. Carrière, S. Pigeot-Rémy, A. Casanova, J.-C. Lazzaroni, C. Guillard, N. Herlin-Boime. Impact of titanium dioxide nanoparticle dispersion state and dispersion method on their toxicity towards A549 lung cells and Escherichia coli bacteria. J Translat. Toxicol., 1(1) : 10-20, 2014.



Maj : 18/04/2014 (1013)


Retour en haut