Salle de cultures cellulaires
David Béal, Sylvain CAILLAT
Salle de cultures cellulaires

photo: D.Michon-Sté ARTECHNIQUE

Les cultures cellulaires sont réalisées dans des milieux nutritifs spécifiques, à 37°C sous atmosphère contenant du CO2. La manipulation des échantillons se fait en milieu stérile, sous hottes à flux laminaire, pour éviter les cotaminations extérieures. Pour ses études de génotoxicité, le laboratoire Lésions des Acides Nucléiques fait essentiellement de la culture de cellules humaines. Celles-ci sont principalement des lignées telles que les monocytes (THP1), hépatocytes (HepG2), cellules pulmonaires (A549), HeLa, ainsi que des cultures primaires de cellules de peau humaine (fibroblastes, kératinocytes, mélanocytes).

 

Maj : 08/09/2014 (411)

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