tdouki.jpg

Thierry DOUKI

laboratoire Systèmes Moléculaires et nanoMatériaux pour l'Energie et la Santé (SyMMES) UMR CNRS/UGA/CEA
équipe Chimie en Interfac avec la Biologie en Environnement, Santé et Toxicologie (CIBEST)

tel.jpg 04.38.78.31.91
fax.png 04.38.78.50.90
email.gif

Fonctions :

Chercheur, Adjoint du chef de Service

Directeur de Recherche du CEA

 

Thèmes de recherche

- Caractérisation des dommages de l'ADN induits par des agents chimiques (hydrocarbures aromatiques polycycliques, agents alkylants, espèces réactives de l'oxygène) ou des rayonnements (ultraviolets, ionisants).

- Développement d'outils analytiques pour la quantification de ces dommages

- Etude de la formation et de la réparation au niveau cellulaire après exposition à divers stress

CV


Formation 

- Habilitation à Diriger les Recherches Université Grenoble I (1999)

- Thèse de Chimie Université Grenoble I (1992)

- Ingénieur de l'Ecole Supérieure de Chimie Inductrielle de Lyon (1989)

- DEA de Chimie Organique Fine Université Lyon I (1989)

Parcours professionel

- 2013-2016 : chef du Service de Chimie Inorganique et Biologique

- 2007-2012: responsable du laboratoire "Lésions des Acides Nucléiques"

- 1993-2007 : chercheur au laboratoire "Lésions des Acides Nucléiques"

- 1992-1993 : stage post-doctoral à l'université de Californie à Berkeley; laboratoire du Prof. Bruce ames
                    (utilisation de lésions de l'ADN comme biomarqueurs de processus endogènes)

- 1989-1992 : thèse de science, dirigée par Jean Cadet (laboratoire LAN)
                     (étude de la photochimie de composés modèle et de l'ADN isolé)

Prix scientifiques
- 1994 : Prix de Jeune Chercheur de la Société Française de Photobiology

- 2006 : "New Investigator Award" de l'American Society for Photobiology

- 2018 : Research Award de l'American Society for Photobioly

 

 

Enseignement

Interventions en M1 et M2 sur la cancérogénèse chimique, les effets du rayonnement UV,

la spectrométrie de masse et le stress oxydant

 

Divers

- Editeur associé de Photochemistry and Photobiology

- Membre de la société Européenne de Photobiologie (comité exécutif 2005-2007)

- Membre de la Sociétés Américaine de Photobiologie (membre du conseil depuis 2014)

- Secrétaire de la Société Française de Photobiologie

- Membre de la Société française de Toxicologie Génénétique

- Membres du Comité d'Experts Scientifique "Agents Physiques" de l'ANSES

      

Publications scientifiques

Co-auteur de plus de 180 articles originaux, et d'une soixantaine d'articles de revue et de chapitres de livre (H-index 61, ISI WoK).

List of publications.pdf

 

sélection de publications  :

(1) Youssef, A., von Koschembahr, A., Caillat, S., Corre, S., Galibert, M. D. and Douki, T. (2018) 6-Formylindolo[3,2-b]carbazole (FICZ) is a Very Minor Photoproduct of Tryptophan at Biologically Relevant Doses of UVB and Simulated Sunlight. Photochem. Photobiol. in press.

(2) von Koschembahr, A., Youssef, A., Béal, D., Calissi, C., Bourgart, E., Marques, M., Leccia, M.-T., Giot, J.-P., Maitre, A. and Douki, T. (2018) Solar simulated light exposure alters metabolization and genotoxicity induced by benzo[a]pyrene in human skin. Sci. Rep. In Press.

(3) Douki, T., Corbiere, C., Preterre, D., Martin, P. J., Lecureur, V., Andre, V., Landkocz, Y., Pottier, I., Keravec, V., Fardel, O., Moreira-Rebelo, S., Pottier, D., Vendeville, C., Dionnet, F., Gosset, P., Billet, S., Monteil, C. and Sichel, F. (2018) Comparative study of diesel and biodiesel exhausts on lung oxidative stress and genotoxicity in rats. Environ. Pollut. 235, 514-524.

(4) Douki, T., von Koschembahr, A. and Cadet, J. (2017) Insight in DNA repair of UV-induced pyrimidine dimers by chromatographic methods. Photochem. Photobiol. 93, 207-215.

(5) Genies, C., Jullien, A., Lefebvre, E., Revol, M., Maitre, A. and Douki, T. (2016) Inhibition of the formation of benzo a pyrene adducts to DNA in A549 lung cells exposed to mixtures of polycyclic aromatic hydrocarbons. Toxicol. Vitro 35, 1-10.

(6) Douki, T. and Sage, E. (2016) Dewar valence isomers, the third type of environmentally relevant DNA photoproducts induced by solar radiation. Photochem. Photobiol. Sci. 15, 24-30.

(7) Douki, T. (2016) Sunlight-induced DNA damage: molecular mechanisms and photoprotection strategies, In Skin Stress Response Pathways: Environmental Factors and Molecular Opportunities (Wondrak, G. T., Ed.) pp 49-77, Springer Int. Publish.

(8) Douki, T. (2016) Relative Contributions of UVB and UVA to the Photoconversion of (6-4) Photoproducts into their Dewar Valence Isomers. Photochem. Photobiol. 92, 587-594.

(9) Banyasz, A., Esposito, L., Douki, T., Perron, M., Lepori, C., Improta, R. and Markovitsi, D. (2016) Effect of C5-Methylation of Cytosine on the UV-Induced Reactivity of Duplex DNA: Conformational and Electronic Factors. J. Phys. Chem. B 120, 4232-4242.

(10) Premi, S., Wallisch, S., Mano, C. M., Weiner, A. B., Bacchiocchi, A., Wakamatsu, K., Bechara, E. J. H., Halaban, R., Douki, T. and Brash, D. E. (2015) Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure. Science 347, 842-847.

(11) Douki, T., Meador, J. A., Berard, I. and Wack, A. (2015) N4-Methylation of Cytosine Drastically Favors the Formation of (6-4) Photoproducts in a TCG Context. Photochem. Photobiol. 91, 102-108.

(12) Batal, M., Rebelo-Moreira, S., Hamona, N., Bayle, P. A., Mouret, S., Clery-Barraud, C., Boudry, I. and Douki, T. (2015) A guanine-ethylthioethyl-glutathione adduct as a major DNA lesion in the skin and in organs of mice exposed to sulfur mustard. Toxicol. Lett. 233, 1-7.

(13) Bacqueville, D., Douki, T., Duprat, L., Rebelo-Moreira, S., Guiraud, B., Dromigny, H., Perier, V., Bessou-Touya, S. and Duplan, H. (2015) A new hair follicle-derived human epidermal model for the evaluation of sunscreen genoprotection. J. Photochem. Photobiol. B: Biol. 151, 31-38.

(14) Meador, J. A., Baldwin, A. J., Pakulski, J. D., Jeffrey, W. H., Mitchell, D. L. and Douki, T. (2014) The significance of the Dewar valence photoisomer as a UV radiation-induced DNA photoproduct in marine microbial communities. Environ. Microbiol. 16, 1808-1820.

(15) Batal, M., Boudry, I., Mouret, S., Clery-Barraud, C., Wartelle, J., Berard, I. and Douki, T. (2014) DNA damage in internal organs after cutaneous exposure to sulphur mustard. Toxicol. Appl. Pharmacol. 278, 39-44.

(16) Genies, C., Maitre, A., Lefebvre, E., Jullien, A., Chopard-Lallier, M. and Douki, T. (2013) The Extreme Variety of Genotoxic Response to Benzo a pyrene in Three Different Human Cell Lines from Three Different Organs. PLoS One 8, 11.

(17) Batal, M., Boudry, I., Mouret, S., Wartelle, J., Emorine, S., Bertoni, M., Berard, I., Clery-Barraud, C. and Douki, T. (2013) Temporal and spatial features of the formation of DNA adducts in sulfur mustard-exposed skin. Toxicol. Appl. Pharmacol. 273, 644-650.

(18) Vaya, I., Gustavsson, T., Douki, T., Berlin, Y. and Markovitsi, D. (2012) Electronic: Excitation Energy Transfer between Nucleobases of Natural DNA. J. Am. Chem. Soc. 134, 11366-11368.

(19) Tarantini, A., Maitre, A., Lefebvre, E., Marques, M., Rajhi, A. and Douki, T. (2011) Polycyclic aromatic hydrocarbons in binary mixtures modulate the efficiency of benzo a pyrene to form DNA adducts in human cells. Toxicology 279, 36-44.

(20) Tarantini, A., Douki, T., Personnaz, M. B., Besombes, J. L., Jafrezzo, J. L. and Maitre, A. (2011) Effect of the chemical composition of organic extracts from environmental and industrial atmospheric samples on the genotoxicity of polycyclic aromatic hydrocarbons mixtures. Toxicol Environ. Chem. 93, 941-954.

(21) Noonan, F. P., Zaidi, M. R., Wolnicka-Glubisz, A., Anver, M. R., Bahn, J., Wielgus, A., Cadet, J., Douki, T., Mouret, S., Popratiloff, A., Merlino, G. and De Fabo, E. C. (2011) Two UV pathways to melanoma. Pigment Cell Melanoma Res. 24, 799-799.

(22) Mouret, S., Leccia, M. T., Bourrain, J. L., Douki, T. and Beani, J. C. (2011) Individual Photosensitivity of Human Skin and UVA-Induced Pyrimidine Dimers in DNA. J. Invest. Dermatol. 131, 1539-1546.

(23) Mouret, S., Bogdanowicz, P., Haure, M. J., Castex-Rizzi, N., Cadet, J., Favier, A. and Douki, T. (2011) Assessment of the Photoprotection Properties of Sunscreens by Chromatographic Measurement of DNA Damage in Skin Explants. Photochem. Photobiol. 87, 109-116.

(24) Banyasz, A., Vaya, I., Changenet-Barret, P., Gustavsson, T., Douki, T. and Markovitsi, D. (2011) Base Pairing Enhances Fluorescence and Favors Cyclobutane Dimer Formation Induced upon Absorption of UVA Radiation by DNA. J. Am. Chem. Soc. 133, 5163-5165.

(25) Mouret, S., Philippe, C., Gracia-Chantegrel, J., Banyasz, A., Karpati, S., Markovitsi, D. and Douki, T. (2010) UVA-induced cyclobutane pyrimidine dimers in DNA: a direct photochemical mechanism? Org. Biomol. Chem. 8, 1706-1711.

(26) Falletti, O. and Douki, T. (2008) Low glutathione level favors formation of DNA adducts to 4-hydroxy-2(E)-nonenal, a major lipid peroxidation product. Chem. Res. Toxicol. 21, 2097-2105.

(27) Falletti, O., Cadet, J., Favier, A. and Douki, T. (2007) Trapping of 4-hydroxynonenal by glutathione efficiently prevents formation of DNA adducts in human cells. Free Radic. Biol. Med. 42, 1258-1269.

(28) Mouret, S., Baudouin, C., Charveron, M., Favier, A., Cadet, J. and Douki, T. (2006) Cyclobutane pyrimidine dimers are predominant DNA lesions in whole human skin exposed to UVA radiation. Proc. Natl. Acad. Sci. U. S. A. 103, 13765-13770.

(29) Douki, T., Ravanat, J. L., Pouget, J. P., Testard, I. and Cadet, J. (2006) Minor contribution of direct ionization to DNA base damage induced by heavy ions. Int. J. Radiat. Biol. 82, 119-127.

(30) Douki, T. (2006) Low ionic strength reduces cytosine photoreactivity in UVC-irradiated isolated DNA. Photochem. Photobiol. Sci. 5, 1045-1051.

(31) Douki, T. (2006) Effect of denaturation on the photochemistry of pyrimidine bases in isolated DNA. J. Photochem. Photobiol. B: Biol. 82, 45-52.

(32) Chandor, A., Berteau, O., Douki, T., Gasparutto, D., Sanakis, Y., Ollagnier-De-Choudens, S., Atta, M. and Fontecave, M. (2006) Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis. J. Biol. Chem. 281, 26922-26931.

(33) Douki, T., Reynaud-Angelin, A., Cadet, J. and Sage, E. (2003) Bipyrimidine photoproducts rather than oxidative lesions are the main type of DNA damage involved in the genotoxic effect of solar UVA radiation. Biochemistry 42, 9221-9226.

 

 

 

 

 

 

 

Retour en haut