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Dr Jean-luc RAVANAT, Ph. D.

SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé

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Scientific Interest

DNA damage and Repair, Chemistry and Biochemistry of DNA & RNA

Radiation Biology, Photobiology, Oxidative stress, Environnmental Toxicology, Nanotoxicology, Radiosensitization

Mechanisms of DNA damage formation, Detection of cellular DNA damages, epigenetic DNA modifications

 

Background

Licence in Biochemistry (1989, Montpellier, France)

Master in Organic Chemistry (1989, Grenoble, France)

Ph. D. in Organic Chemistry (1992, Grenoble, France)

Habilitation (HRD) (1999, Grenoble, France)

 

Experience

1989-1992 : Ph. D. student, laboratory "Lésions des Acides Nucléiques", CEA Grenoble (Supervisor Dr J. Cadet).

1993-1995 : Post-doctoral fellow, Laboratory "Metabolism of Xenobiotics", Quality and Safety Assurance Department, Nestlé Rserach Centre, Switzerland.

1995-2016 : Research Scientist, CEA Grenoble, Laboratory "Lésions des Acides Nucléiques"

2012-2013 : Head of the laboratory "Lésions des Acides Nucléiques"

since 2017 : Research Scientist laboratory "Chimie Interface Biologie, pour l'Environnement, la Santé et la Toxicology"

Since 2011 : Research Director at CEA.

 

Competences

 

Chemical synthesis (modified DNA lesions, including isotopically labeled compouds)

Analytical chemistry (HPLC, GC, Immunoaffinity chromatography,...) with special interest in HPLC coupled through electrospray ionization to tandem mass spectrometry using isotopically labeled internal standards.

Spectroscopy : One and two diemnsional NMR (1H, 13C, 15N), mass spectrometry (ESI-ions trap, Maldi-TOF) , FT-IR,...

Biochemistry : Endo- and Exo-nucleases, DNA repair enzymes, monoclonal antibodies, single cell gel electrophoresis (comet assay), cell culture.

Award

"Young investigator award" delivered by the International Society for Free Radical Resarch, Barcelona, Spain 1996

Scientific activities

Involved in the evaluation processes of several scientific journals : J. Am. Chem. Soc, Nat. Methods., Chem. Res. Toxicol., Photochem. Photobiol., Free Rad Biol. Med., Radiat. Res., Int. J. Radiat. Biol., New J. Chem., Photochem. Photobiol. Sci., Rapid Commun. Mass. Spec., Cancer Sci., Biochemistry, Anal. Chem., J Phys. Chem., Mutat. Res. …

Co-authored of more than 160 publications (H-index 54) and 26 book chapters.

 

List of principal recent publications

DNA Damage formation & Repair

Gomez Mendoza, M., Banyasz, A., Douki, T., Markovitsi, D. and Ravanat, J.L. (2016) Direct Oxidative Damage of Naked DNA Generated upon Absorption of UV Radiation by Nucleobases. J. Phys. Chem. Lett., 7, 3945-3948.

Dumont, E., Grüber, R., Bignon, E., Morell, C., Randa, J., Ravanat, J.-L. and Tunon, I. (2016) Singlet oxyen attack on guanine: reactivity and structural signature within the B-DNA helix. Chem. Eur. J., 22, 12358_62.

Dumont, E., Gruber, R., Bignon, E., Morell, C., Moreau, Y., Monari, A. and Ravanat, J.L. (2016) Probing the reactivity of singlet oxygen with purines. Nucleic Acids Res., 44, 56-62.

Barbier, E., Lagorce, A., Hachemi, A., Dutertre, M., Gorlas, A., Morand, L., Saint-Pierre, C., Ravanat, J.L., Douki, T., Armengaud, J., Gasparutto, D., Confalonieri, F. and Breton, J. (2016) Oxidative DNA damage and repair in the radioresistant archaeon Thermococcus gammatolerans. Chem. Res. Toxicol. 29, 1796-1809.

 

Silerme, S., Bobyk, L., Taverna-Porro, M., Cuier, C., Saint-Pierre, C. and Ravanat, J.L. (2014) DNA-polyamine crosslinks generated upon one electron oxidation of DNA. Chem Res Toxicol, 27, 1011-1018.

Radiosensitization

Bayart, E., Pouzoulet, F., Calmels, L., Dadoun, J., Allot, F., Plagnard, J., Ravanat, J.L., Bridier, A., Denoziere, M., Bourhis, J. and Deutsch, E. (2017) Enhancement of IUdR Radiosensitization by Low-Energy Photons Results from Increased and Persistent DNA Damage. PLoS One, 12, e0168395.

Taupin, F., Flaender, M., Delorme, R., Brochard, T., Mayol, J.F., Arnaud, J., Perriat, P., Sancey, L., Lux, F., Barth, R.F., Carriere, M., Ravanat, J.L. and Elleaume, H. (2015) Gadolinium nanoparticles and contrast agent as radiation sensitizers. Phys. Med. Biol., 60, 4449-4464.

Bobyk, L., Edouard, M., Deman, P., Vautrin, M., Pernet-Gallay, K., Delaroche, J., Adam, J.F., Esteve, F., Ravanat, J.L. and Elleaume, H. (2013) Photoactivation of gold nanoparticles for glioma treatment. Nanomedicine, 9, 1089-97.

Review articles

Ravanat, J.L. and Douki, T. (2016) UV and ionizing radiations induced DNA damage, differences and similarities. J. Rad. Phys. Chem. , 128, 92-102.

Ferreri, C., Golding, B.T., Jahn, U. and Ravanat, J.L. (2016) COST ACTION CM1201 "Biomimetic Radical Chemistry": free radical chemistry successfully meets many disciplines. Free Radic Res, 1-40.

Carriere, M., Sauvaigo, S., Douki, T. and Ravanat, J.L. (2016) Impact of nanoparticles on DNA repair processes: current knowledge and working hypotheses. Mutagenesis.

Nikitaki, Z., Hellweg, c.E., Georgakilas, A.G. and Ravanat, J.-L. (2015) Stress-induced DNA Damage biomarkers: Applications and limitations. Frontiers in Chemistry, 3.

Cadet, J., Douki, T. and Ravanat, J.L. (2015) Oxidatively generated damage to cellular DNA by UVB and UVA radiation. Photochem Photobiol, 91, 140-155.

Ravanat, J.L., Breton, J., Douki, T., Gasparutto, D., Grand, A., Rachidi, W. and Sauvaigo, S. (2014) Radiation-mediated formation of complex damage to DNA: a chemical aspect overview. Br. J. Radiol., 87, 20130715.

Ravanat, J.-L., Cadet, J. and Douki, T. (2012) Oxidatively generated DNA lesions as potential biomarkers of in vivo oxidative stress. Curr. Mol. Med., 12, 655-674.

Complete list of publications (updated January 2017)

Publications JLRavanat.pdf

 

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