tdouki.jpg

Thierry DOUKI

laboratory Molecular Systems and Nanomaterials for Energy and Health(SyMMES) UMR CNRS/UGA/CEA
team Chemistry at the interface with Biology in Environment, Health and Toxicology (CIBEST)

tel.jpg 04.38.78.31.91
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Functions:

Scientisit, deputy head of the laboratory

 

Research Interests

- Characterization of DNA damage induced by chemical agents (Polycyclic aromatic hydrocarbons, alkylating agents, agents alkylants, reactive oxygen species) and radiation (ultraviolet, ionizing).

- Development of analytical tools for the quantification of these damage.

- Study of the formation and repair at the cellular level after exposure to various stresses.

 

CV

 


Training
- HDR, Grenoble University (1999)

- PhD in chemistry, Grenoble University (1992)
- Diploma of the Chemistry Engineering School of Lyon (1989)
- Master in organic synthesis, Lyon University (1989)

Parcours professionel
- since2013: head of the Laboratory of Biological and Inorganic Chemsitry

- 2007-2012: Head of the "Nucleic Acids Lesions" team

- 1993-2007: permanent scientist in the "Nucleic Acids Lesions" team
- 1992-1993: post-doctoral fellow at University of California in Berkeley; Prof. Bruce Ames' group
                    (DNA damage as biomarkers of endogenous processes)
- 1989-1992: Science thesis, supervised by Jean Cadet ("Nucleic Acids Lesions" team)
                     (Study of the photochemistry of isolated DNA and of model compounds)

Prix scientifiques
- 1994: Young scientist award of the French Society for Photobiology
- 2006: "New Investigator Award" of the AmericanSociety for Photobiology

 

      

Publications

Co-author of more than 170 original articles , and of more than 60 review articicles and book chapters (H-index 56).

 

Selection of publications:

  1. T. Douki, A. Reynaud, J. Cadet & E. Sage (2003) Bipyrimidine photoproducts rather than oxidative lesions are the main type of DNA damage involved in the genotoxic effect of solar UVA radiation. Biochemistry 42, 9221-9226.
  2. S. Courdavault, C. Baudouin, M. Charveron, B. Canghilem, A. Favier, J. Cadet & T. Douki (2005) Repair of the three main types of bipyrimidine DNA photoproducts in human keratinocytes exposed to UVB and UVA radiations. DNA Repair 4, 836-844.
  3. T. Douki, J.-L. Ravanat, J.-P. Pouget, I. Testard & J. Cadet (2006) Minor contribution of direct ionization to DNA base damage induced by heavy ions, Int. J. Radiat. Biol. 82, 119-127.
  4. S. Mouret, C. Baudouin, M. Charveron, A. Favier, J. Cadet & T. Douki (2006) Cyclobutane pyrimidine dimers are predominant DNA lesions in whole human skin exposed to UVA radiation. Proc. Natl. Acad. Sci. USA 103, 13765-13770
  5. O. Falletti; J. Cadet, .A. Favier & T. Douki (2007) Trapping of 4-hydroxynonenal by glutathione efficiently prevents formation of DNA adducts in human cells. Free Radic. Biol. Med. 42, 1258-1269.
  6. S. Mouret, M. Charveron, A. Favier, J. Cadet & T. Douki (2008) Differential repair of UVB-induced cyclobutane pyrimidine dimers in cultured human skin cells and whole human skin. DNA Repair 7, 704-712
  7. A. Tarantini, A. Maitre, E. Lefebvre, M. Marques, C. Marie, J.-L. Ravanat & T. Douki (2009) Relative contribution of DNA strand breaks and DNA adducts to the genotoxicity of benzo[a]pyrene as a pure compound and in complex mixtures. Mutat. Res. 671, 67–75
  8. Vaya, I., Gustavsson, T., Miannay, F. A., Douki, T. & Markovitsi, D. (2010) Fluorescence of natural DNA: From the femtosecond to the nanosecond time scale. J. Am. Chem. Soc. 132, 11834-11835.
  9. S. Mouret, M.-T. Leccia, J.-L. Bourrain, T. Douki &J.-C. Beani (2011) Individual photosensitivity of human skin and UVA-induced pyrimidine dimers in DNA. J. Invest. Dermatol. 131, 1539–1546
  10. A. Tarantini, T. Douki, M.-B. Personnazc, J.-L. Besombes, J.-L. Jafrezzo & A. Maître (2011) Effect of the chemical composition of organic extracts from environmental and industrial atmospheric samples on the genotoxicity of Polycyclic Aromatic Hydrocarbons mixtures. Toxicol. Environ. Chem. 93, 941-954
  11. A. Banyasz, I. Vayá, P. Changenet-Barret, T. Gustavsson, T. Douki & D. Markovitsi (2011) Base-pairing enhances fluorescence and favors cyclobutane dimer formation induced upon absorption of UVA radiation by DNA, J. Am. Chem. Soc. 133, 5163-5165.
  12. S. Mouret, A. Forestier & T. Douki (2012) The specificity of UVA-induced DNA damage in human melanocytes, Photochem. Photobiol. Sci. 11, 155-162.
  13. Noonan, F. P., Zaidi, M. R., Wolnicka-Glubisz, A., Anver, M. R., Bahn, J., Wielgus, A., Cadet, J., Douki, T., Mouret, S., Tucker, M. A., Popratiloff, A., Merlino, G. and De Fabo, E. C. (2012) Melanoma induction by ultraviolet A but not ultraviolet B radiation requires melanin pigment. Nature Comm. 3.
  14. C. Genies, A. Maître, E. Lefèbvre, A. Jullien, M. Chopard-Lallier & T. Douki (2013) The extreme variety of genotoxic response to benzo[a]pyrene in three different human cell lines from three different organs, PLOS One 8, e78356.
  15. M. Batal, I. Boudry, S. Mouret, J. Wartelle, S. Emorine, M. Bertoni, I. Bérard, C. Cléry-Barraud, and T. Douki (2013) Temporal and spatial features of the formation of DNA adducts in sulphur mustard-exposed skin, Toxicol. Appl. Pharmacol. 273, 644–650.
  16. M. Batal, I. Boudry, S. Mouret, C. Cléry-Barraud, J. Wartelle, I. Bérard & T. Douki (2014) DNA damage in internal organs after cutaneous exposure to sulphur mustard, Toxicol. Appl. Pharmacol. 278, 39-44
  17. T. Douki, I. Bérard, A. Wack & S. Andrä (2014) Contribution of cytosine-containing cyclobutane dimers to DNA damage produced by photosensitized triplet-triplet energy transfer, Chem. Eur. J. 19, 5787-5794
  18. J. A. Meador, A. J. Baldwin, J. D. Pakulski, W. H. Jeffrey, D. L. Mitchell & T. Douki (2014) The significance of the Dewar valence photoisomer as an ultraviolet radiation induced DNA photoproduct in marine microbial communities, Environm. Microbiol. 16, 1808–1820
  19. L. Esposito, A. Banyasz, T. Douki, M. Perron, D. Markovitsi and R. Improta (2014) Effect of C5-methylation of cytosine on the photoreactivity of DNA: a joint experimental and computational study of TCG trinucleotides. J. Am. Chem. Soc. 136, 10838-10841
  20. T. Douki, J. A. Meador, I. Bérard and A. Wack (2015) N4-Methylation of cytosine drastically favors the formation of (6-4) photoproducts in a TCG context, Photochem. Photobiol. 91, 102-108.
  21. M. Batal, S. Rebelo-Moreira, N. Hamon, P.-A. Bayle, S. Mouret, C. Cléry-Barraud, I. Boudry and T. Douki (2015) A guanine-ethylthioethyl-glutathione adduct as a major DNA lesion in the skin and in organs of mice exposed to sulphur mustard, Toxicol. Lett. 233, 1-7
  22. T. Douki, S. Rebelo-Moreira, N. Hamon and P.-A Bayle (2015) DNA photochemistry: geometrically unconstrained pyrimidine (6-4) pyrimidone photoproducts do photoisomerize, Org. Lett. 17, 246-249
  23. S. Premi, S. Wallisch, C. M. Mano, A. B. Weiner, A. Bacchiocchi, K. Wakamatsu, E. J. H. Bechara, R. Halaban, T. Douki and Douglas E. Brash (2015) Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure. Science 347, 842-847
  24. D. Bacqueville, T. Douki, L. Duprat, S. Rebelo-Moreira, B. Guiraud, H. Dromigny, V. Perier, S. Bessou-Touya, H. Duplan (2015) A new hair follicle-derived human epidermal model for the evaluation of sunscreen genoprotection, J. Photochem. Photobiol. B: Biol. 151 31-38.
  25. T. Douki (2016) Relative contributions of UVB and UVA to the photoconversion of (6-4) photoproducts into their Dewar valence isomers. Photochem. Photobiol. 92, 586-594.
  26. C. Genies, A. Jullien, E. Lefebvre, M. Revol, A. Maitre and T. Douki (2016) Inhibition of the formation of benzo[a]pyrene adducts to DNA in A549 lung cells exposed to mixtures of polycyclic aromatic hydrocarbons. Toxicol. In vitro 35, 1-10.

 

 

 

Teaching

Lectures on chemical carcinogenesis, effects of UV radiation, mass spectrometry and oxidative stress

 

Other

 

- Associate editor for Photochemistry and Photobiology
- Member of the executive committee of the European Society for Photobiology (2005-2007)
- Member of the American and European Societies for Photobiology

- Secretary of the French Society for Photobiology

- Member of the French Society for Genetic Toxicology

- Expert in the "Physical Agents" group of the National Agency of Heatlh and Environmental Safety (ANSES)

 

 

 

 

Liste de publications TDouki.pdf

 

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