Spectroscopy of Metalloproteins
Active Sites of Metalloproteins
I am mainly interested in studies of metalloproteins from both the structural and functional point of view by using two complementary spectroscopic methods: EPR and NMR
Ferredoxins, such the one presented below, exemplify particularly well the various properties of the proteins which can be examined.
By EPR: (at low temperatures ~4 K)
number of active sites (2),
oxidation state (Ox or red),
oxidation states of the particular Fe ions (FeIII or FeII),
redox potential (titration),
nature of the magnetic coupling (ferro or anti-ferro) inside the active site
and between the active sites (exchange or dipolar)……..
By NMR: (at ambient temperature)
examination of nucleus surrounding the active site (1H, 13C, .)which are probes References (as exemples):
P.Kyritsis et al. Biochemistry (1999) 38, 6335-6345
R. Kümmerle et al. J. Biol. Inorg. Chem. (2001) 6 : 446-451
Last update : 06/14 2005 (154)