Dernière mise à jour : 22-08-2017

INAC

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• Cellular biology, physiology and cellular imaging

 

Epigenetic Cytosine Modifications In Leukemia

SL-DRF-17-0825

Research field : Cellular biology, physiology and cellular imaging
Location :

SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SyMMES)

Laboratoire Lésions des Acides Nucléiques

Grenoble

Contact :

Jean BRETON

Starting date : 01-10-2017

Contact :

Jean BRETON

Université Grenoble Alpes - DRF/INAC/SyMMES/CIBEST

04-38-78-56-01

Thesis supervisor :

Jean BRETON

Université Grenoble Alpes - DRF/INAC/SyMMES/CIBEST

04-38-78-56-01

Cytosine methylation is an epigenetic process playing an important role in gene expression regulation. Recently, it has been proven that this mechanism is more complicated than expected. Indeed, other cytosine modifications have been identified in DNA: 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC), and 5-carboxycytosine (5-caC).

Carcinogenesis is often associated to changes in the proportion and distribution of cytosine epigenetic modifications in DNA. This work will be focused on MyeloDysplatic Syndrome (MDS) and Acute Myeloid Leukemia (AML). Using cellular models and patients samples, we will: (i) measure and compare 5-mC, 5-hmC, 5-fC and 5-caC levels in different forms of MDS and AML. These rates will be obtained globally for whole DNA, and in specific gene regulation sequences. We will also measure the expression of key genes involved in DNA methylation and demethylation. (ii) Using the same approaches, we will study the influence of therapeutic agents on epigenetic cytosine modifications.

These experiments should help us to improve our understanding of carcinogenesis and responses to anticancer treatements.

 

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