Arnaud BUHOT

Structure et Propriétés d'Architectures Moléculaires
Equipe Chimie pour la Reconnaissance et l'Etude des Assemblages Biologiques



Since March 2002 : CEA research engineer

January 2007 - February 2014: Head of the Theory Group

July 2014 : Head of the CREAB


  Proposition de stage Master 2 ou Thèse

Puces à Aptamères

Contacts : A. Buhot ( )


  Reserch interests

Theory of microarrays (DNA or protein microarrays)

Biopolymer conformation

DNA based architectures

Soft Condensed Matter

Lectures on Numerical Simulations in Statistical Mechanics

Plateforme BIOMADE (BIOhybrid MAterial Design and Engineering)


Since July 2014: Head of the CREAB

Jan. 2007 - Feb. 2014: Head of the Theory Group

Since March 2002: Research Engineer, SPrAM, INAC, CEA Grenoble

Jan. 2000 - Jan. 2002: Marie Curie Fellowship, Theoretical Physics, Oxford

Oct. 1999 - Jan. 2000: Postdoctoral Associate, Theoretical Physics, Oxford (EPSRC)

July 1998 - Sept. 1999: Research Associate, SPSMS, DRFMCCEA Grenoble (AMN)

May 1999: PhD in Physics, Université Joseph Fourier of Grenoble

Aug. 1997 - June 1998: Research Assistant (in lieu of Mulitary Duty), LPS, ENS Paris

Sept. 1996 - Jul 1997: Research Associate, SPSMS, DRFMC, CEA Grenoble (AMN)

Sept. 1992 - Aug. 1996: Student at the Ecole Normale Supérieure of Lyon

  Scientific publications


Real time observation and automated measurement of red blood cells agglutination inside a passive microfluidic biochip containing embedded reagents
M Huet, M Cubizolles, A Buhot
Biosensors and Bioelectronics, 2016
Polymers translocation through nano-pores in vibrating thin membranes
T Menais, S Mossa, A Buhot
Scientific Reports, 6, 38558, 2016
A nanoparticle-based thermo-dynamic aptasensor for small molecule detection
F Melaine, C Coilhac, Y Roupioz, A Buhot
Nanoscale, 8, 16947-16954, 2016
M Jourdan, J Pingel, A Buhot, T Livache, JF Constant
Materials Science, 2(4): 473-483, 2015
Biophysics of DNA: DNA Melting Curve Analysis with Surface Plasmon Resonance Imaging
A Buhot, J Pingel, JB Fiche, R Calemczuk, T Livache
Introduction to Plasmonics: Advances and Applications, 61-88, 2015
On the use of aptamer microarrays as a platform for the exploration of human prothrombin/thrombin conversion
C Daniel, Y Roupioz, T Livache, A Buhot
Analytical biochemistry, 473, 66-71, 2015
Landscapes of taste
LA Garcon, M Genua, Y Hou, A Buhot, R Calemczuk, D Bonnaffe, H Lortat-Jacob, T Livache, Y Hou
CHEMICAL SENSES 40 (3), 244-245 (2015)
Gold Nanoparticles Surface Plasmon Resonance Enhanced Signal for the Detection of Small Molecules on Split-Aptamer Microarrays
F Melaine, Y Roupioz, A Buhot
Microarrays, 4 (1), 41-52, 2015
SPR imaging based electronic tongue via landscape images for complex mixture analysis
M Genua, LA Garçon, V Mounier, H Wehry, A Buhot, M Billon, R Calemczuk, D Bonnaffé, Y Hou, T Livache
Talanta, 130, 49-54, 2014
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
Y Hou, M Genua, LA Garçon, A Buhot, R Calemczuk, D Bonnaffé, H Lortat-Jacob, T Livache
JoVE (Journal of Visualized Experiments), e51901-e51901, 2014
LA Garçon, Y Hou, M Genua, A Buhot, R Calemczuk, D Bonnaffé, Y Hou, T Livache
Sensor Letters, 12 (6-7), 1059-1064, 2014
C Brakha, P Arvers, F Villiers, A Marlu, A Buhot, T Livache, R Calemczuk, JP Zarski, CL Villiers, PN Marche, MB Villiers
SpringerPlus, 3 (1), 56, 2014
C Daniel, Y Roupioz, D Gasparutto, T Livache, A Buhot
PloS one, 8 (9), e75419, 2013
A Harrison, H Binder, A Buhot, CJ Burden, E Carlon, C Gibas, LJ Gamble, A Halperin, J Hooyberghs, DP Kreil, R Levicky, PA Noble, A Ott, BM Pettitt, D Tautz, AE Pozhitkov
Nucleic acids research, 41 (5), 2779-2796, 2013
Real time monitoring of thrombin interactions with its aptamers: insights into the sandwich complex formation
C Daniel, F Mélaïne, Y Roupioz, T Livache, A Buhot
Biosensors and Bioelectronics, 40 (1), 186-192, 2013
Y Hou, M Genua, D Tada Batista, R Calemczuk, A Buhot, P Fornarelli, J Koubachi, D Bonnaffé, E Saesen, C Laguri, H Lortat‐Jacob, T Livache 
Angewandte Chemie, 124 (41), 10540-10544, 2012
J Liu, P Pham, V Haguet, F Sauter-Starace, L Leroy, A Roget, E Descamps, A Bouchet, A Buhot, P Mailley, T Livache
Analytical chemistry, 84 (7), 3254-3261, 2012
J Pingel, A Buhot, R Calemczuk, T Livache
Biosensors and Bioelectronics,  31 (1), 554-557, 2012
S Milgram, S Cortes, MB Villiers, P Marche, A Buhot, T Livache, Y Roupioz
Biosensors and Bioelectronics, 26 (5), 2728-2732, 2011
CBM du Puch, E Barbier, A Kraut, Y Couté, J Fuchs, A Buhot, T Livache, M Sève, A Favier, T Douki, D Gasparutto, S Sauvaigo, J Breton
Archives of biochemistry and biophysics, 507 (2), 296-303, 2011
MB Villiers, A Buhot, C Brakha, C Marquette, PN Marche 
INTECH In: Schab-Balcerzak E (ed) Electropolymerization. 199-214, 2011

Viscosity and Renewal Times of Polymer Reptation Models

A. Buhot

Macromolecules 43, 9155-9159 (2010).


Salt Concentration Effects on Equilibrium Melting Curves from DNA Microarrays

J. Fuchs, J. B. fiche, A. Buhot, R. Calemczuk, T. Livache

Biophys. J. 99, 1886-1895 (2010).


Effects of formamide on the thermal stability of DNA duplexes on biochips

J. Fuchs, D. Dell'Atti, A. Buhot, R. Calemczuk, M. Mascini and T. Livache

Anal. Biochem. 397, 132-134 (2010)


SPR imaging for label-free multiplexed analyses of DNA N-glycosylase interactions with damaged DNA duplexes

C. Corne, J.B. Fiche, D. Gasparutto, V. Cunin, E. Suraniti, A. Buhot, J. Fuchs, R. Calemczuk, T. Livache and A. Favier

Analyst 133, 1036-1045 (2008)


Point Mutation Detection by Surface Plasmon Resonance Imaging Coupled with a Temperature Scan Method in a Model System

J. B. Fiche, J. Fuchs, A. Buhot, R. Calemczuk, and T. Livache

Anal. Chem. 80, 1049-1057 (2008)


Stretching of homopolymeric RNA reveals single-stranded helices and base-stacking

Y. Seol, G. Skinner, K. Visscher, A. Buhot, and A. Halperin

Phys. Rev. Lett. 98, 158103 (2007)

Physics News Update, 829-2 (2007)


Temperature effects on DNA Chip Experiments from Surface Plasmon Resonance imaging: Isotherms and Melting Curves

J. B. Fiche, A. Buhot, R. Calemczuk, and T. Livache

Biophys. J. 92, 935-946 (2007) 

Faculty of 1000 Biology evaluation


Hybridization at a Surface: The Role of Spacers in DNA Microarrays

A. Halperin, A. Buhot and E. B. Zhulina

Lagnmuir 22, 11290-11304 (2006)  


On the Hybirdization Isotherms of DNA Microarrays: The Langmuir Model and its Extensions

A. Halperin, A. Buhot and E. B. Zhulina

J. Phys. Condens. Matter 18, S463-S490 (2006)


Exact curvilinear diffusion coefficients in the repton model

A. Buhot

Eur. Phys. J. E 18, 239-244 (2005) 


Brush Effects on DNA Chips: Thermodynamics, Kinetics and Design Guidelines

A. Halperin, A. Buhot and E. B. Zhulina

Biophys. J. 89, 796-811 (2005)  


Cluster algorithm for nonadditive hard-core mixtures

A. Buhot

J. Chem. Phys. 122, 024105 (2005)


Hybridization Isotherms of DNA Microarrays and the Quantification of Mutation Studies

A. Halperin, A. Buhot and E. B. Zhulina

Clin. Chem. 50, 2254-2262 (2004)


Effects of stacking on the configurations and elasticity of single-stranded nucleic acids

A. Buhot and A. Halperin

Phys. Rev. E 70, 020902(R) (2004)


Sensitivity, specificity and the hybridization isotherms of DNA chips

A. Halperin, A. Buhot and E. B. Zhulina  

Biophys. J. 86, 718-730 (2004)


Kovacs effect and fluctuation-dissipation relations in 1D kinetically constrained models

A. Buhot

J. Phys. A: Math. Gen. 36, 12367-12377 (2003)


Simple strong glass forming models: mean-field solution with activation

A. Buhot, J. P. Garrahan and D. Sherrington

J. Phys. A: Math. Gen. 36, 307-328 (2003)


Fluctuation-Dissipation Relations in the Activated Regime of Simple Strong-Glass Models

A. Buhot and J. P. Garrahan

Phys. Rev. Lett. 88, 225702 (2002)


Extension Behavior of Helicogenic Polypeptides

A. Buhot and A. Halperin

Macromolecules 35, 3238-3252 (2002)


Crossover from fragile to strong glassy behaviour in the spin facilitated chain model

A. Buhot and J. P. Garrahan

J. Phys.: Condens. Matter 14, 1499-1507 (2002)


Glassy behaviour in simple kinetically constrained models: topological networks, lattice analogues and annihilation-diffusion

D. Sherrington, L. Davison, A. Buhot and J. P. Garrahan

J. Phys.: Condens. Matter 14, 1673-1682 (2002)


Rigorous Bounds to Retarded Learning

A. Buhot, M. B. Gordon and J. P. Nadal

Phys. Rev. Lett. 88, 099801 (2002)

Comment to D. Herschkowitz and M. Opper, Phys. Rev. Lett. 86, 2174 (2001) 


Crossover from Fragile to Strong Glassy Behaviour in Kinetically Constrained Systems

A. Buhot and J. P. Garrahan

Phys. Rev. E 64, 021505 (2001)


Glassy behaviour in a 3-state spin model

L. Davison, D. Sherrington, J. P. Garrahan and A. Buhot

J. Phys. A: Math. Gen. 34, 5147-5182 (2001)


Robust learning and generalization with Support Vector Machines

A. Buhot and M. B. Gordon

J. Phys. A: Math. Gen. 34, 4377-4388 (2001)


On the Helix-Coil transition in grafted chains

A. Buhot and A. Halperin

 Europhys. Lett. 50, 756-761 (2000)


Storage capacity of a constructive learning algorithm

A. Buhot and M. B. Gordon  

J. Phys. A: Math. Gen. 33, 1713-1727 (2000)


The Extension of Rod-Coil Multiblock Copolymers and the Effect of the Helix-Coil Transition

A. Buhot and A. Halperin

Phys. Rev. Lett. 84, 2160-2163 (2000)


A reply to the Comment by A. A. Louis on  Phys. Rev. Lett. 82, 960 (1999) 

A. Buhot

Phys. Rev. Lett. 84, 1841 (2000)


Phase separation in two-dimensional additive mixtures

A. Buhot and W. Krauth

Phys. Rev. E 59, 2939-2941 (1999)


Packing fraction at the phase separation transition in hard-core mixtures

A. Buhot

Phys. Rev. Lett. 82, 960-963 (1999)


Bayesian learning versus optimal learning

M. B. Gordon and A. Buhot

Physica A 257, 85-98 (1998)


Numerical Solution of Hard-Core Mixtures

A. Buhot and W. Krauth

Phys. Rev. Lett. 80, 3787-3790 (1998)

Phys. Rev. Focus 1, story 11, May 4, 1998


Phase transitions in optimal unsupervised learning

A. Buhot and M. B. Gordon

Phys. Rev. E 57, 3326-3333 (1998)


Finite size scaling of the Bayesian perceptron

A. Buhot, J.-M. Torres-Moreno and M. B. Gordon

Phys. Rev. E 55, 7434-7440 (1997)


Cost function and patterns distribution of the bayesian perceptron

A. Buhot and M. B. Gordon

Phys. Lett. A228, 73-78 (1997) 


Precise study of some number fields and Galois actions occurring in conformal field theory

E. Buffenoir, A. Coste, J. Lascoux, P. Degiovanni, A. Buhot  

Annales de l'institut Henri Poincaré (A) Physique théorique, 63, 41-79 (1995)


Storage Capacity of the Tilinglike Learning Algorithm
A. Buhot and M. B. Gordon
Proceedings of the Conference Disordered and Complex Systems, pages 17-22,
King's College, London, July 2000

Statistical mechanics of support vector machines
A. Buhot and M. B. Gordon
Proceedings ESANN'99, 201-206 (1999)

Detection of two Gaussian clusters
A. Buhot and M. B. Gordon
Proceedings ESANN'99, 327-332 (1999)

Numerical simulations of an optimal algorithm for supervised learning
A. Buhot, J.-M. Torres-Moreno and M. B. Gordon
Proceedings ESANN'97, 151-156 (1997)


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