Yoann ROUPIOZ, PhD
Senior Scientist for the CNRS
Laboratory "Structure and Properties of Molecular Assemblies"
Team "Chemistry for Recognition and Studies of Biological Assemblies"
- Researcher for the CNRS (Centre National pour la Recherche Scientifique) since 2005
- Deputy Head of SPrAM since 2014
- Deputy Head of SyMMES laboratory since 2016
- Deputy Head of GDR Bio-engineering of Interfaces since 2016
- Member of the Scientific Advisory Board of Labex ARCANE A Bio-driven Chemistry since 2012
- Member the Organizing Comitee of the European School On Nanosciences & Nanotechnologies ESONN
- Scientific expert for Prestodiag since 2012
- Surface Functionalisation
- Development of Cell Biochips, Bacteria Biochips
- Cellular Monitoring on-the-chip
- Surface treatments ensuring bio-compatibility
- Aptamer-based Biochips
After Yoann ROUPIOZ completed his undergraduate studies in Biochemistry at the University Joseph Fourier (UJF) in 1995 (Grenoble, France), he was recipient of a ‘UJF Scholarship’ as well as an International Scholarship from the Région Rhône-Alpes to pursue his graduate studies at the University of Montreal (Canada) where he graduated from the Chemistry Department (1997). His research interest at this time was “the chemical synthesis of non-natural peptides for the elucidation of enzymatic mechanisms”. In 1998, after completing his obligatory military service with the French Alpine Troops, he chose to return to France to study in the laboratory of Prof. J. Lhomme, where he received a Ligue Nationale de Lutte contre le Cancer grant to complete a PhD in the field of Nucleic Acids lesions and DNA repair studies. In 2001, further received a one-year funding from the French Foreign Office (‘Lavoisier’ grant) to undertake post-graduate studies at the University of British Columbia (Prof. D. M. Perrin group, Vancouver, Canada) where he developed original approaches for DNAzymes selection based on modified nucleic acids. Following this, Yoann undertook additional post-doc training in the group of Dr X. Gidrol at the French Nuclear Energy Office (near Paris) where he focused on living cell micro-arrays. Since 2005, Yoann has been a permanent Researcher for the French National Center for Scientific Research (CNRS) and is developing cell-based biosensors and biochips in Grenoble. In 2007, he was recipient of the ‘young Researcher Award’ of the French Biochemical and Molecular Biology Society and also recipient of the 2011 Masao International Award in Kyoto, Japan. He has also been a member of the 'Nano and micro technologies for Biology' strategic watch group of the OMNT from 2008 to 2013.
Projects currently in progess
- Biochips for individualized cell studies. This project is led in collaboration with the LAAS in Toulouse, FRANCE. We developed an original access to surfaces patterned by several proteins to ensure multicell-type capture on a single micro-array.
- Aptamer biochips. Aptamers are short DNA sequences having a strong and specific affinity for a target. Using Surface Plasmon Resonance imaging (SPRi), we develop biochips capable of Thrombine concentration monitoring at concentrations biological relevant. We also have an other aptamer-based project running which is focused on the combination of Gold nanoparticles with Surface Plasmon Resonance detection for the monitoring of low levels of targets.
- Development of Biochips and Biosensors for pathogenic bacterial detection (label-free monitoring). These projects are led in close collaboration with the Prestodiag biotechnology company.
Scientific publications (since 2005)
- Florian Mallevre et al. (2016) Real-time toxicity testing of silver nanoparticles to Salmonella Enteritidis using surface plasmon resonance imaging: A proof of concept, NanoImpact 1, 55–59
- Templier V, Roux A, Roupioz Y, & Livache T (2016) Ligands for label-free detection of whole bacteria on biosensors: a review. Trends in Analytical Chemistry in press.
- Mondani L, et al. (2016) Fast detection of both o157 and non-o157 shiga-toxin producing escherichia coli by real-time optical immunoassay. Lett Appl Microbiol.62, 39-46.
- Melaine F, Roupioz Y, & Buhot A (2015) Gold Nanoparticles Surface Plasmon Resonance Enhanced Signal for the Detection of Small Molecules on Split-Aptamer Microarrays (Small Molecules Detection from Split-Aptamers). Microarrays 4:41-52.
- Daniel C, Roupioz Y, Livache T, & Buhot A (2015) On the use of aptamer microarrays as a platform for the exploration of human prothrombin/thrombin conversion. Analytical biochemistry 473:66-71.
- Baganizi DR, et al. (2015) A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip. Biosensors 5:750-767.
- Roupioz, Y. (2014) Cell-Based Microarrays: Review of Applications, Developments and Technological Advances. Palmer, E., Ed. Springer Science & Business Media; p 110.
- Mondani, L.; Roupioz, Y.; Delannoy, S.; Fach, P.; Livache, T. (2014), Simultaneous enrichment and optical detection of low levels of stressed Escherichia coli O157:H7 in food matrices. J Appl Microbiol, 117, 537-46.
- Laplatine L, et al. (2014) Spatial resolution in prism-based Surface Plasmon Resonance, Optics Express, 22, 22771-85.
- Leroy L, et al. (2014) Photothermal effect for localized desorption of primary lymphocytes arrayed on an antibody/DNA-based biochip. Lab Chip. 14(12):1987-1990
- Mondani L, et al. (2014) Simultaneous enrichment and optical detection of low levels of stressed Escherichia coli O157:H7 in food matrices. J Appl Microbiol (2):537-546
- Bendali A, et al. (2014) Cell specific electrodes for neuronal network reconstruction and monitoring. Analyst; 139(13):3281-3289
- Daniel C, et al. (2013) Solution-phase vs surface-phase aptamer-protein affinity from a label-free kinetic biosensor. PLoS One; 8(9):e75419. doi: 10.1371/journal.pone.0075419.
- Bouguelia S, et al. (2013) On-chip microbial culture for the specific detection of very low levels of bacteria. Lab Chip; 13(20):4024-4032
- Liu J, et al. (2013) Selective Individual Primary Cell Capture Using Locally Bio-Functionalized Micropores. Plos One ; 8(3) :e57717
- Daniel C, et al. (2013) Real time monitoring of thrombin interactions with its aptamers: Insights into the sandwich complex formation. Biosensors & bioelectronics 40(1):186-192.
- Milgram S, et al. (2012) Antibody microarrays for label-free cell-based applications. Methods 56(2):326-333.
- Bombera R, et al. (2012) DNA-directed capture of primary cells from a complex mixture and controlled orthogonal release monitored by SPR imaging. Biosensors & bioelectronics 33(1):10-16.
- Roupioz Y, et al. (2011) Blood cell capture on antibody microarrays and monitoring of the cell capture using surface plasmon resonance imaging. Methods Mol Biol 706:139-149.
- Milgram S, et al. (2011) On chip real time monitoring of B-cells hybridoma secretion of immunoglobulin. Biosensors & bioelectronics 26(5):2728-2732.
- Roupioz Y, et al. (2009) Individual Blood-Cell Capture and 2D Organization on Microarrays. Small 5(13):1493-1497.
- Milgram S, et al. (2009) Real-time and label-free analysis of cellular activity on chip. IEEE Sensors Journal (25-28 Oct. 2009):1768-1771.
- Suraniti E, et al. (2007) Real-time detection of lymphocytes binding on an antibody chip using SPR imaging. Lab on a chip 7(9):1206-1208
- "Biosensor" option, at the UJF (Master M1 and M2 degree) (since 2006)
- "Molecular Biology for Biochips & Biosensors", Auckland University, New Zealand (2009)
- "Molecular Biology and dedicated techniques", "Microarrays, Biosensors & Biochips", Instituto Politecnico Nacional, Mexico City, Mexico (2010)
- Institut de la Vision, Paris, FRANCE
- LAAS, Toulouse, FRANCE
- Institut Albert Bonniot, Grenoble, FRANCE
- Institut de Biologie Structurale, Grenoble, FRANCE
- GenOptics, Orsay, FRANCE
- CEA-DSV, Saclay, FRANCE
- IFREMER, Brest, FRANCE
- Groupe de physique théorique, CEA-Grenoble, FRANCE
Awards and Fellowships
- Grant for Foreign Graduate Training from the 'Région Rhône-Alpes' (1996)
- Grant for Graduate Studies from the 'Université Joseph Fourrier' (1996)
- PhD Grant from the French 'Ligue Nationale de Lutte contre le Cancer' (1998-2001)
- Post-doctoral fellowship from the 'French Foreign Office' (Lavoisier Program)
- 'Young Researcher Prize' from the 'French Society of Molecular Biology and Biochemistry' (2007)
- Masao Horiba Award 2011, Kyoto, Japan
Contracts - Financial supports
- Prise de Risque à l'interface Physique-Chimie-Biologie (CNRS) : Projet "Réseaux bioniques" (2010-2011)
- ANR Blanc 2010 : Projet "Mult-I-Cell-S" (2011-2014)
- ANR P2N 2011 : Projet "Cellestim" (2011-2015)
- Techno Santé CEA : Projet "Elispri" (2010-2011) and "Salmoscreen" (2012-2014)
- Labex ARCANE : Projet "Cellsecret" (2012-2014)
- RTRA Fondation Nanosciences (Grenoble)